Team:Paris-Saclay/Project/Notebook/Week 9

From 2012.igem.org

Follow us !

Week 9

30th july

  • Analysis of the Petri dishes after the transformation with the B construction
Electrophoresis of BBa_K274100 and pSB1A2 on a 0.8% Agarose gel.
Week9-1.jpg


31th July

  • Miniprep of the colonies B1 and B2 that came from the transformation with the B construction.
  • Nanovue to measure the concentration of the samples B1, B2 and the control


Week9-2.jpg
  • Amplification by PCR of the 880bp end of BBa_K098995 contained in the colonies B1, B2 and test on the control and amplification of the whole B construction.
Week9-3.jpg

PCR program used:

Week9-4.jpg